| Autor: |
Jaimes-Hoy, Lorraine, Pérez-Maldonado, Adrián, Bahena, Elian Narváez, Guarneros, Natalia De La Cruz, Rodríguez-Rodríguez, Adair, Charli, Jean-Louis, Soberón, Xavier, Joseph-Bravo, Patricia |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.14195585.v1 |
| Popis: |
The hypothalamus-pituitary-thyroid (HPT) axis regulates energy balance through the pleiotropic action of thyroid hormones. HPT basal activity and stimulation by cold or voluntary exercise are repressed by previous chronic stress in adults. Maternal separation (MS) modifies HPT basal activity we thus studied the response of the axis to energy demands and analyzed possible epigenetic changes on Trh promoter. Non-handled (NH) or MS male Wistar rats were cold exposed 1h at adulthood, only in NH rats Trh expression in the hypothalamic paraventricular nucleus (PVN) and serum TSH concentration were increased. Two weeks of voluntary exercise decreased fat mass, increased Trh expression, and thyroid hormones concentration changed proportionally to running distance in NH male rats and MS male rats. Although NH-females ran more than MS and much more than males, exercise decreased body weight and fat mass only in NH-rats with no change on any parameter of HPT axis, but increased Pomc expression in arcuate-nucleus of NH- whereas Npy in MS-females. Overall methylation pattern of PVN Trh gene promoter was similar in NH-males or -females; MS modified methylation of specific CpG sites, a thyroid-hormone receptor (THR) binding site present after the initiation site was hypomethylated in MS-males; in MS-females, the THR binding-site of the proximal promoter (site 4) and two sites in the first intron were hypermethylated. Our studies showed that, in a sex dimorphic manner, MS blunted the responses of HPT axis to energy demands in adult animals and caused methylation changes on Trh promoter that could alter T3 feedback. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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