Development and evaluation of monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay for the diagnosis of acute leptospirosis in humans

Autor:	Jiro Arikawa, Virajie Weerakkodi, Yomani D. Sarathkumara, Kumiko Yoshimatsu, Kanae Shiokawa, Chandika D. Gamage
Rok vydání:	2018
Předmět:	0301 basic medicine medicine.drug_class 030106 microbiology 030231 tropical medicine Immunology Enzyme-Linked Immunosorbent Assay Monoclonal antibody Sensitivity and Specificity Antigen capture 03 medical and health sciences Antibodies Monoclonal Murine-Derived 0302 clinical medicine Antigen Immunology and Allergy Medicine Humans Leptospirosis Operational costs chemistry.chemical_classification Antigens Bacterial biology business.industry medicine.disease Virology Antibodies Bacterial Enzyme chemistry Monoclonal biology.protein Antibody Leptospira interrogans business
Zdroj:	Journal of immunological methods. 463
ISSN:	1872-7905
Popis:	Monoclonal antibody (MAb)-based ELISA was developed to detect leptospiral antigens from the plasma of febrile patients. The MAb reacts specifically with pathogenic leptospires and the assay possesses excellent diagnostic test parameters compared to PCR, indicating that this new ELISA is useful for the early diagnosis of leptospirosis with low operational cost.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26322cc1f041dfd4896f5bc76cbbe1f9 https://pubmed.ncbi.nlm.nih.gov/30217719 Zobrazit plný text záznamu Full Text from ScienceDirect