The effect of high glucose on polyol pathway activity and myoinositol metabolism in cultured human endothelial cells
| Autor: | K. Bartlett, K. G. M. M. Alberti, C. S. Hetherington, G. C. Hawthorne |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Umbilical Veins
medicine.medical_specialty Endothelium Endocrinology Diabetes and Metabolism chemistry.chemical_compound Polyol pathway Polyol Internal medicine Lactate dehydrogenase Internal Medicine medicine Humans Sorbitol Incubation Cells Cultured chemistry.chemical_classification L-Lactate Dehydrogenase Metabolism Endothelial stem cell Kinetics Glucose Endocrinology medicine.anatomical_structure chemistry Endothelium Vascular Inositol |
| Zdroj: | Diabetologia. 32:163-166 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/bf00265088 |
| Popis: | Cultured human endothelial cells were incubated with high glucose (27.5 mmol/l) supplemented medium for 0, 2, 6, 12, 24 and 48 h. Lactate dehydrogenase was assayed as a measure of cell number. Sorbitol and myoinositol were determined as trimethyl silyl derivatives using gas chromatography mass spectrometry. Sorbitol, after an initial lag phase of 2 h, accumulated rapidly to plateau at 2 to 3 fold control value by 24 h, no further increase being seen over a further 24 h (Baseline, 1.31 +/- 0.36, 24 h 3.50 +/- 0.74 pmol sorbitol/unit lactate dehydrogenase; p less than 0.001). There was no change in myoinositol levels over the 24 h period. Sorbitol accumulation occurred at glucose concentration greater than 18.1 mmol/l and only returned to baseline levels after 24 h of incubation in normal medium (glucose levels 5.9 mmol/l). Sorbitol levels are increased by incubation of endothelial cells at high glucose levels but myoinositol levels remain unchanged. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink