Analysis of Subforms of Free Prostate-Specific Antigen in Serum by Two-Dimensional Gel Electrophoresis: Potential to Improve Diagnosis of Prostate Cancer
| Autor: | Janett Reiche, Dietmar Schnorr, Axel Boehme, Carsten Stephan, Klaus Jung, S. A. Loening, Pranav Sinha, Wolfgang Hoesel |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pathology medicine.medical_specialty Clinical Biochemistry Prostatic Hyperplasia Urology Sensitivity and Specificity law.invention Prostate cancer Antigen law medicine Humans Electrophoresis Gel Two-Dimensional Immunoadsorption Aged Chemiluminescence Two-dimensional gel electrophoresis biology business.industry Biochemistry (medical) Prostatic Neoplasms Reproducibility of Results Blood Proteins Middle Aged Prostate-Specific Antigen medicine.disease Blood proteins Prostate-specific antigen biology.protein Antibody business Protein Binding |
| Zdroj: | Clinical Chemistry. 50:2292-2301 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/clinchem.2004.040469 |
| Popis: | Background: The aim of this study was to develop a method to separate and quantify subforms of free prostate-specific antigen (fPSA) in serum by two-dimensional electrophoresis and to assess the diagnostic accuracy of these subforms for prostate cancer (PCa) diagnosis in comparison with total PSA (tPSA) and the ratio of fPSA to tPSA (%fPSA). Methods: Sera from 50 patients with and without PCa, respectively, were studied. PSA was isolated by immunoadsorption on streptavidin-coated magnetic beads with biotinylated anti-PSA antibodies and separated by two-dimensional electrophoresis. After semidry blotting, the intensities of the fPSA spots were quantified by chemiluminescence using an imager analyzer. Results: The method detected subforms to a concentration of 0.1 μg/L fPSA with an imprecision (CV) 15%. There were no associations between the PCa grading scale and fPSA subforms. Conclusions: fPSA subforms separated by two-dimensional electrophoresis may improve both sensitivity and specificity in prostate cancer diagnostics compared with tPSA and %fPSA. The development of a practicable assay based on the immunologic properties of these different fPSA subforms seems to be promising. |
| Databáze: | OpenAIRE |
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