Characterization of the Murine Myeloid Precursor Cell Line MuMac-E8
| Autor: | Jens Knauer, Frank Emmrich, Sina Riemschneider, Doris Wolf, Jörg Lehmann, Stephan Fricke, Cathleen Pfefferkorn, Christiane Fueldner, Nadja Hilger, Janine Kohlschmidt, Ulrich Sack |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2014 |
| Předmět: |
Immune Cells
Cell Lines Cellular differentiation Immunology lcsh:Medicine Biology Research and Analysis Methods Stem cell marker Cell morphology White Blood Cells Animal Cells Medicine and Health Sciences lcsh:Science Induced pluripotent stem cell Molecular Biology Cell potency Blood Cells Multidisciplinary Macrophages Stem Cells lcsh:R Biology and Life Sciences Transformed Cell Lines Cell Biology Hematology Cell biology Endothelial stem cell lcsh:Q Biological Cultures Cellular Types Stem cell Stem Cell Lines Research Article Adult stem cell |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 12, p e113743 (2014) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0113743 |
| Popis: | Starting point for the present work was the assumption that the cell line MuMac-E8 represents a murine cell population with stem cell properties. Preliminary studies already pointed to the expression of stem-cell associated markers and a self-regenerative potential of the cells. The cell line MuMac-E8 should be examined for their differential stage within stem cell hierarchy. MuMac-E8 cells were derived from a chimeric mouse model of arthritis. It could be shown that MuMac-E8 cells express mRNA of some genes associated with pluripotent stem cells (Nanog, Nucleostemin), of genes for hematopoietic markers (EPCR, Sca-1, CD11b, CD45), for the mesenchymal marker CD105 and of genes for the neural markers Pax-6 and Ezrin. In methylcellulose and May-Grünwald-Giemsa staining, hematopoietic colonies were obtained but the hematopoietic system of lethally irradiated mice could not be rescued. Osteogenic differentiation was not detectable. Thus, it became evident that MuMac-E8 represents not a stem cell line. However, MuMac-E8 cells expressed several myeloid surface markers (i.e. CD11b, F4/80, CD14, CD64), showed phagocytosis and is capable of producing nitric oxide. Thus, this cell line seems to be arrested an advanced stage of myeloid differentiation. Adherence data measured by impedance-based real-time cell analysis together with cell morphology data suggested that MuMac-E8 represents a new macrophage precursor cell line exhibiting weak adherence. This cell line is suitable as an in-vitro model for testing of macrophage functions. Moreover, it might be also useful for differentiation or reprogramming studies. |
| Databáze: | OpenAIRE |
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