The Role of Citrate in the Regulation of Hepatic Fatty Acid Synthesis by Insulin and Glucagon
| Autor: | Marion G. J. Schmitz, M.J.H. Geelen |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biology Biochemistry Glucagon Citric Acid chemistry.chemical_compound Enzyme activator Cytosol Endocrinology Internal medicine medicine Animals Insulin Citrates Rats Wistar Fatty acid synthesis Pancreatic hormone Fatty Acids Biochemistry (medical) Acetyl-CoA carboxylase General Medicine Rats Pyruvate carboxylase Enzyme Activation Liver chemistry sense organs Caprylates Citric acid Acetyl-CoA Carboxylase Subcellular Fractions |
| Zdroj: | Hormone and Metabolic Research. 25:525-527 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-2007-1002166 |
| Popis: | Incubation of intact rat hepatocytes with insulin and glucagon resulted in increased and decreased rates of de novo fatty acid biosynthesis, respectively. These changes were paralleled by corresponding alterations in the activity of acetyl-CoA carboxylase, an important regulatory enzyme of this pathway. The hormonal conditions imposed on the hepatocytes did not change the cellular or the cytosolic level of citrate. Incubation of hepatocytes with octanoate showed a parallel increase in the rate of fatty acid synthesis and acetyl-CoA carboxylase with a concomitant elevation of the cellular citrate level. The increase in whole-cell citrate was mainly due to a marked increase in the level of cytosolic citrate. Collectively, our data indicate that insulin and glucagon-determined changes in acetyl-CoA carboxylase are not mediated by changes in cytosolic citrate levels. |
| Databáze: | OpenAIRE |
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