Enzyme-responsive multifunctional surfaces for controlled uptake/release of (bio)molecules
| Autor: | Gian Luca De Gregorio, Giuseppe Gigli, Laura Blasi, Mariangela Mortato, Simona Argentiere |
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| Přispěvatelé: | Mortato, Mariangela, Argentiere, Simona, De Gregorio Gian, Luca, Gigli, Giuseppe, Blasi, Laura |
| Rok vydání: | 2014 |
| Předmět: |
Streptavidin
Proteases Biotin Nanotechnology Biocompatible Materials Cathepsin D chemistry.chemical_compound Colloid and Surface Chemistry Drug/biomolecules delivery Tissue engineering Physical and Theoretical Chemistry chemistry.chemical_classification Tissue Engineering Biomolecule Surfaces and Interfaces General Medicine Controlled release Multifunctional Enzymes Enzyme-responsive surfaces chemistry Covalent bond Biotinylation Drug delivery Streptavidin-biotin interaction Biotinylated depolymerized chitosan Biosensor Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces (Online) 123 (2014): 89–95. doi:10.1016/j.colsurfb.2014.08.034 info:cnr-pdr/source/autori:Mortato M.; Argentiere S.; De Gregorio G.L.; Gigli G.; Blasi L./titolo:Enzyme-responsive multifunctional surfaces for controlled uptake%2Frelease of (bio)molecules/doi:10.1016%2Fj.colsurfb.2014.08.034/rivista:Colloids and surfaces. B, Biointerfaces (Online)/anno:2014/pagina_da:89/pagina_a:95/intervallo_pagine:89–95/volume:123 |
| ISSN: | 1873-4367 |
| DOI: | 10.1016/j.colsurfb.2014.08.034 |
| Popis: | The current trend in the development of biomaterials is towards bioactive and biodegradable systems. In particular, enzyme-responsive structures are useful tools to realize biodegradable surfaces for the controlled delivery of biomolecules/drugs through a triggered surface erosion process. Up to now, enzyme-responsive structures have been designed by covalent linkage between synthetic polymers and biodegradable functionalities that are responsive to chemical and biological cues (i.e. proteases or pH) [1], [2], [3] and [4]. Here, we present a novel approach to achieve enzyme-responsive surface-attached networks by exploiting the non-covalent interaction between streptavidin and biotin. The functional component of this three-dimensional (3D) structure is a layer of biotinylated peptides that are degraded by the action of specific proteases. The system was stable under typical physiological conditions; however, it was efficiently degraded upon enzyme exposure. Further, the controlled release of biomolecules and drugs – previously entrapped into the surface-attached network – was demonstrated to occur as a consequence of the enzymatic cleavage. This versatile approach does not require complex chemical procedures. Interestingly, it can be easily adapted to different enzyme-peptide partners and therefore is very attractive for tissue replacement, drug delivery and biosensing. |
| Databáze: | OpenAIRE |
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