Characterization of melA: a gene encoding melanin biosynthesis from the marine bacterium Shewanella colwelliana
| Autor: | Daniel C. Stein, Vernon E. Coyne, Chun-Mean Lin, Ronald M. Weiner, W. C Fuqua |
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| Rok vydání: | 1991 |
| Předmět: |
Reading Frames
Transcription Genetic Tyrosinase DNA Mutational Analysis Molecular Sequence Data Reading frame Marine Biology Molecular cloning Biology Protein Sorting Signals Start codon Sequence Homology Nucleic Acid Gram-Negative Bacteria Genetics Escherichia coli Animals Amino Acid Sequence Cloning Molecular Gene Peptide sequence Gene Library Melanins Binding Sites Base Sequence Monophenol Monooxygenase Nucleic acid sequence General Medicine Molecular biology Ostreidae Open reading frame Tyrosine Copper |
| Zdroj: | Gene. 109(1) |
| ISSN: | 0378-1119 |
| Popis: | A recombinant plasmid with the ability to impart melanin synthesis to an Escherichia coli host was isolated from a Shewanella colwelliana genomic library. The genetic determinant of the Mel+ phenotype is carried on a 1.3-kb DNA fragment and sequence analysis of this revealed a single intact open reading frame that was sufficient for melanin synthesis (mel). This gene is expressed as a monocistronic transcript and a putative transcription start point is located 115 nucleotides upstream from the translational start codon. The mel gene encoded a protein of 39.5 kDa [346 amino acids (aa)] that showed no aa sequence homology with other proteins known to mediate melanin synthesis (e.g., tyrosinases). |
| Databáze: | OpenAIRE |
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