Deciphering the Rules of Ribosome Binding Site Differentiation in Context Dependence

Autor:	Yanting Duan, Xiaojuan Zhang, Weiji Zhai, Jinpeng Zhang, Xiaomei Zhang, Guoqiang Xu, Hui Li, Zhaohong Deng, Jinsong Shi, Zhenghong Xu
Rok vydání:	2022
Předmět:	Binding Sites Bacteria Base Sequence Protein Biosynthesis Biomedical Engineering Escherichia coli General Medicine Biochemistry Genetics and Molecular Biology (miscellaneous) Ribosomes
Zdroj:	ACS synthetic biology. 11(8)
ISSN:	2161-5063
Popis:	The ribosome binding site (RBS) is a crucial element regulating translation. However, the activity of RBS is poorly predictable, because it is strongly affected by the local possible secondary structure, that is, context dependence. By the Flowseq technique, over 20 000 RBS variants were sorted and sequenced, and the translation of multiple genes under the same RBS was quantitatively characterized to evaluate the context dependence of each RBS variant in
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2621365401ef017f9e1482df60e065be https://pubmed.ncbi.nlm.nih.gov/35877551 Zobrazit plný text záznamu