Enzyme Hinders HIV-1 Tat Viral Transport and Real-Time Measured with Nanopores
| Autor: | Yunjiao Wang, Deqiang Wang, Yin Bohua, Liu Qianshan, Wenli Huang, Liang Wang, Xiyun Guan, Han Wang, Liyuan Liang, Wei Li |
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| Rok vydání: | 2021 |
| Předmět: |
Fluid Flow and Transfer Processes
chemistry.chemical_classification medicine.diagnostic_test biology Process Chemistry and Technology Proteolysis Bioengineering Transporter Biological Transport Trypsin Transmembrane protein Enzyme assay Nanopores Enzyme chemistry medicine Fluorescence microscope Biophysics biology.protein HIV-1 tat Gene Products Human Immunodeficiency Virus Instrumentation Intracellular medicine.drug |
| Zdroj: | ACS sensors. 6(10) |
| ISSN: | 2379-3694 |
| Popis: | HIV-1 Tat protein, an intercellular transporter with a determinant function of delivering "information-rich" molecules in viral multiplication, was tryptic-hydrolyzed and real-time single molecule-monitored in a transmembrane pore. The electrokinetic studies revealed the catalytic and inhibitory effects on enzymatic digestion associated with Ca2+ and Cu2+ ions, respectively, in response to binding interactions with trypsin. Our strategy permits accurate and distinguishable sensing of Ca2+ and Cu2+via an enzyme assay. In addition, considering the closer mimic of the real situation of HIV spread, measurements in the serum and on cells were also investigated. Transmembrane current measurements together with fluorescence microscopy imaging indicated the potential to perturb the Tat transport in the serum environment and on cells. Because the involved Tat proteolysis should prevent the occurrence of viral delivery, the presented method probably enables efficient hindrance to HIV-1 infection, in complementary to current traditional treatments. |
| Databáze: | OpenAIRE |
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