Quality control methods in musculoskeletal tissue engineering: from imaging to biosensors
| Autor: | Sibylle Grad, Daniele Zuncheddu, Marco Fosca, Julietta V. Rau, Elena Della Bella, Annapaola Parrilli, Gaia Giovanna Maria Rocchitta, Heinz Redl, Silvia Generelli, Mauro Alini, Felix Kurth, Valentina Basoli, Margherita Maioli, Dalila Petta, Sophie Verrier, Andrea Schwab, Pier Andrea Serra |
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| Rok vydání: | 2021 |
| Předmět: |
Histology
QH301-705.5 Physiology Computer science Endocrinology Diabetes and Metabolism media_common.quotation_subject Review Article 02 engineering and technology 03 medical and health sciences Tissue engineering Musculoskeletal tissue QP1-981 Quality monitoring Quality (business) Biology (General) Bone 030304 developmental biology media_common 0303 health sciences Quality control Microcomputed tomography 021001 nanoscience & nanotechnology Bone quality and biomechanics Risk analysis (engineering) Narrative review 0210 nano-technology Control methods |
| Zdroj: | Bone Research, Vol 9, Iss 1, Pp 1-21 (2021) Bone Research |
| ISSN: | 2095-6231 |
| DOI: | 10.1038/s41413-021-00167-9 |
| Popis: | Tissue engineering is rapidly progressing toward clinical application. In the musculoskeletal field, there has been an increasing necessity for bone and cartilage replacement. Despite the promising translational potential of tissue engineering approaches, careful attention should be given to the quality of developed constructs to increase the real applicability to patients. After a general introduction to musculoskeletal tissue engineering, this narrative review aims to offer an overview of methods, starting from classical techniques, such as gene expression analysis and histology, to less common methods, such as Raman spectroscopy, microcomputed tomography, and biosensors, that can be employed to assess the quality of constructs in terms of viability, morphology, or matrix deposition. A particular emphasis is given to standards and good practices (GXP), which can be applicable in different sectors. Moreover, a classification of the methods into destructive, noninvasive, or conservative based on the possible further development of a preimplant quality monitoring system is proposed. Biosensors in musculoskeletal tissue engineering have not yet been used but have been proposed as a novel technology that can be exploited with numerous advantages, including minimal invasiveness, making them suitable for the development of preimplant quality control systems. |
| Databáze: | OpenAIRE |
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