BopC is a type III secreted effector protein of Burkholderia pseudomallei
| Autor: | Sunsiree Muangman, Gunnar N. Schroeder, Veerachat Muangsombut, Gad Frankel, Natalie R. Lazar Adler, Sunee Korbsrisate, Edouard E. Galyov, Varintip Srinon |
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| Rok vydání: | 2011 |
| Předmět: |
Burkholderia pseudomallei
Virulence Factors Recombinant Fusion Proteins Mutant Microbiology Type three secretion system Cell Line Plasmid Bacterial Proteins Genes Reporter Gene cluster Genetics Escherichia coli Humans Secretion Molecular Biology biology Effector Epithelial Cells biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Chaperone (protein) biology.protein bacteria Gene Deletion Molecular Chaperones Protein Binding |
| Zdroj: | FEMS microbiology letters. 323(1) |
| ISSN: | 1574-6968 |
| Popis: | Burkholderia pseudomallei , the causative agent of melioidosis, exploits the Bsa type III secretion system (T3SS) to deliver effector proteins into host cells. These effectors manipulate host cell functions; thus, contributing to the ability of the bacteria to evade the immune response and cause disease. Only two Bsa-secreted effectors have been conclusively identified to date. Here, we report the identification of the third B . pseudomallei type III secreted effector protein, designated BopC. BopC is encoded by the bpss1516 gene abutting, bpss1517 , which encodes its putative chaperone. The genes are located in the close proximity to the bsa T3SS gene cluster of B. pseudomallei K96243 (Fig. 1). BopC was secreted into culture supernatant by the wild-type B . pseudomallei strain, but its secretion was abolished in the bsaZ T3SS mutant. Using pull down and co-purification assays, we confirmed that BopC interacts with its putative chaperone, BPSS1517, in vitro . Furthermore, the first 20 N-terminal amino acids of BopC were found to be sufficient to mediate the T3SS-dependent translocation of a reporter protein from a heterologous enteropathogenic Escherichia coli host into mammalian cells. Finally, bopC mutant was found to be less invasive than the wild-type strain in the epithelial cells. |
| Databáze: | OpenAIRE |
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