Evaluation of X, Y, 18, and 13/21 alpha satellite DNA probes for interphase cytogenetic analysis of uncultured amniocytes by fluorescence in situ hybridization
| Autor: | Valère Cacheux, Jean-François Oury, S. Sérero, C. Nessmann, Gérard Tachdjian, P. Blot, Luc Druart |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty X Chromosome Chromosomes Human Pair 21 Aneuploidy Biology DNA Satellite Y chromosome Pregnancy Prenatal Diagnosis Y Chromosome medicine Humans Prospective Studies Interphase Genetics (clinical) X chromosome In Situ Hybridization Fluorescence Chromosome Aberrations medicine.diagnostic_test Chromosomes Human Pair 13 Hybridization probe Cytogenetics Obstetrics and Gynecology Chromosome Karyotype medicine.disease Amniotic Fluid Molecular biology Karyotyping Female Chromosomes Human Pair 18 DNA Probes Fluorescence in situ hybridization |
| Zdroj: | Prenatal diagnosis. 14(2) |
| ISSN: | 0197-3851 |
| Popis: | The major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. This prospective study evaluated the use of four commercially available centromeric DNA probes (DXZ1, DYZ1, D18Z1, and D13Z1/D21Z1) for direct analysis of uncultured amniocytes. One hundred and sixteen amniotic fluid samples were analysed by FISH and standard cytogenetics. This evaluation demonstrated that FISH with X, Y, and 18 alpha satellite DNA probes could accurately and rapidly detect aneuploidies involving these chromosomes and could be used in any prenatal clinical laboratory. In contrast, the 13/21 alpha satellite DNA probe hybridizing both chromosomes 13 and 21 was unreliable for prenatal diagnosis in uncultured amniocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text