Chromatinization of Escherichia coli with archaeal histones
| Autor: | Kathryn M Stevens, Antoine Hocher, Matthias Merkenschlager, Maria Rojec, Tobias Warnecke |
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| Rok vydání: | 2019 |
| Předmět: |
Life Sciences & Biomedicine - Other Topics
0301 basic medicine Gene Expression Methanothermus fervidus 0601 Biochemistry and Cell Biology Histones chemistry.chemical_compound Transcription (biology) MUTATIONAL ANALYSIS TRANSCRIPTION Biology (General) biology General Neuroscience METHANOTHERMUS-FERVIDUS General Medicine Chromosomes Bacterial Chromosomes and Gene Expression Recombinant Proteins Nucleosomes Chromatin Cell biology Histone Methanobacteriales Medicine GROWTH Life Sciences & Biomedicine Research Article Micrococcal nuclease H-NS EXPRESSION chromosomes QH301-705.5 Science DNA-BINDING PHASE 030106 microbiology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences evolution Escherichia coli Nucleosome Biology Evolutionary Biology Science & Technology GENE-REGULATION General Immunology and Microbiology E. coli Chromosome Promoter TRANSFORMATION 030104 developmental biology chemistry biology.protein chromatin Other DNA |
| Zdroj: | eLife eLife, Vol 8 (2019) |
| ISSN: | 2050-084X |
| Popis: | Nucleosomes restrict DNA accessibility throughout eukaryotic genomes, with repercussions for replication, transcription, and other DNA-templated processes. How this globally restrictive organization emerged during evolution remains poorly understood. Here, to better understand the challenges associated with establishing globally restrictive chromatin, we express histones in a naive system that has not evolved to deal with nucleosomal structures: Escherichia coli. We find that histone proteins from the archaeon Methanothermus fervidus assemble on the E. coli chromosome in vivo and protect DNA from micrococcal nuclease digestion, allowing us to map binding footprints genome-wide. We show that higher nucleosome occupancy at promoters is associated with lower transcript levels, consistent with local repressive effects. Surprisingly, however, this sudden enforced chromatinization has only mild repercussions for growth unless cells experience topological stress. Our results suggest that histones can become established as ubiquitous chromatin proteins without interfering critically with key DNA-templated processes. |
| Databáze: | OpenAIRE |
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