The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
| Autor: | Fernando Carrari, Martine Lemaire-Chamley, Kentaro Mori, Christophe Rothan, Joana Jorly, Hiroshi Ezura, Erika Asamizu, Mariana Conte, Tsuyoshi Mizoguchi |
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| Přispěvatelé: | Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Ryukoku University, International Christian University, Graduate School of Life and Environmental Sciences, University of Tsukuba, University of Tsukuba, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tsukuba = University of Tsukuba, This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan ('Japan-France Joint Laboratory Project'), the Japan Society for the Promotion of Science (JSPS) and the National Institute for Agricultural Research (INRA) (Bilateral Joint Research Project, grant to KM and ML-C), and the Region Aquitaine (Regal project no. 20111201002), and was carried under the auspices of the EU COST QualityFruit FA 1106 |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Arabidopsis thaliana Physiology growth [SDV]Life Sciences [q-bio] Plant Science tomato 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Gene Expression Regulation Plant Auxin Arabidopsis Transcriptional regulation [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Brassinosteroid BIM signalling Transcription factor Plant Proteins 030304 developmental biology 2. Zero hunger chemistry.chemical_classification 0303 health sciences biology food and beverages fruit Plants Genetically Modified biology.organism_classification Cell biology brassinosteroids chemistry Ectopic expression cell expansion Transcription Factors 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2021, 72 (4), pp.1181-1197. ⟨10.1093/jxb/eraa495⟩ |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/eraa495 |
| Popis: | Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling. |
| Databáze: | OpenAIRE |
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