Development of EST‐SSR markers in Saxifraga sinomontana (Saxifragaceae) and cross‐amplification in three related species
| Autor: | Qingbo Gao, Faqi Zhang, Yan Li, Liu-Kun Jia, Zhi-Hua Wang, Shilong Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Saxifraga congestiflora Locus (genetics) Plant Science Biology 010603 evolutionary biology 01 natural sciences Loss of heterozygosity Transcriptome 03 medical and health sciences lcsh:Botany Saxifraga Allele lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics Saxifraga tangutica Genetic diversity Phylogenetic tree Saxifragaceae Saxifraga sinomontana food and beverages biology.organism_classification Saxifraga heleonastes lcsh:QK1-989 030104 developmental biology lcsh:Biology (General) Evolutionary biology EST‐SSR markers transcriptome |
| Zdroj: | Applications in Plant Sciences, Vol 7, Iss 6, Pp n/a-n/a (2019) |
| ISSN: | 2168-0450 |
| Popis: | Premise Saxifraga sinomontana (Saxifragaceae) is a widespread alpine species in the Qinghai-Tibetan Plateau and its flanking mountains. We developed a set of expressed sequence tag-simple sequence repeat (EST-SSR) markers to investigate the genetic diversity and evolutionary history of the species. Methods and results We initially designed 50 EST-SSR markers based on transcriptome data of S. sinomontana. Nineteen of 50 loci (38%) were successfully amplified, 13 of which were polymorphic. These were tested on 71 individuals from four populations. Three to 18 alleles per locus were detected, and the levels of observed and expected heterozygosity ranged from 0.2817 to 0.9155 and 0.2585 to 0.8495, respectively. In addition, cross-amplification was successful for all 13 loci in three congeneric species, S. tangutica, S. heleonastes, and S. congestiflora. Conclusions These EST-SSR markers will be useful for studying the genetic diversity of S. sinomontana and disentangling the phylogenetic relationships of related species. |
| Databáze: | OpenAIRE |
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