Expression pattern of 5'-nucleotidase in Dictyostelium
| Autor: | Can M. Eristi, Charles L. Rutherford, Muatasem Ubeidat |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Embryology
Slug Genes Protozoan Molecular Sequence Data Biology Gene Expression Regulation Enzymologic 5'-nucleotidase Animals Genetically Modified Genes Reporter Nucleotidase Animals Dictyostelium Promoter Regions Genetic Gene 5'-Nucleotidase Regulation of gene expression Reporter gene fungi Gene Expression Regulation Developmental Artificial Gene Fusion biology.organism_classification beta-Galactosidase Molecular biology Lac Operon Developmental Biology |
| Zdroj: | Mechanisms of development. 110(1-2) |
| ISSN: | 0925-4773 |
| Popis: | In order to analyze the expression pattern of the 5'-nucleotidase (5nt) gene in Dictyostelium, we made a fusion construct in which the 5nt promoter directed the expression of beta-galactosidase gene. The reporter gene was not active in vegetative amoebae but was expressed during the aggregation stage. At the slug stage, 5nt was highly expressed in pstAB cells. As the slug moved along the substratum, high activity of beta-galactosidase was detected in cells that were left behind in the slime trail. In the completed fruiting body, 5nt was expressed in the lower cup, the anterior like cells (ALC) and the basal disc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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