Decorin inhibits nucleus pulposus apoptosis by matrix‐induced autophagy via the mTOR pathway

Autor: Hui-Ren Wang, Tai-Wei Zhang, Wang Ding, Jian Dong, Ze-Fang Li, Guan-Jie Han, Libo Jiang, Xilei Li, Sheng-Long Ding
Rok vydání: 2020
Předmět:
Zdroj: Journal of Orthopaedic Research. 39:1777-1788
ISSN: 1554-527X
0736-0266
Popis: Decorin (Dcn) is a member of the class I small leucine-rich proteoglycans, whose expression in the nucleus pulposus (NP) of intervertebral discs (IVDs) has been shown to increase with aging in humans and sheep. Dcn induces autophagy in endothelial cells; however, its precise role in NP and IVD degeneration during aging is not well understood. We addressed this question in the present study by treating rat nucleus pulposus cells (NPCs) with different concentrations of Dcn. The Western blot analysis and terminal deoxynucleotidyl transferase dUTP nick end labeling assay results showed that Dcn treatment induced autophagy and decreased apoptosis caused by interleukin (IL)-1β application. This effect was dependent on the protein kinase B/mechanistic target of rapamycin (mTOR)/p70 S6 kinase signaling. Dcn treatment also decreased the expression of matrix metalloproteinase-3 and -13 and decreased the IL-1β-induced attenuation of collagen type II and aggrecan levels. The role of Dcn in stimulating autophagy was further supported by the fact that the observed effects were abrogated by knocking down autophagy-related protein 7 with Atg7 small interfering RNA. Thus, Dcn protects NPCs in IVDs from IL-1β-induced apoptosis and degeneration by promoting autophagy through mTOR signaling.
Databáze: OpenAIRE
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