Macro- and microelements in the rat liver, kidneys, and brain tissues; sex differences and effect of blood removal by perfusion in vivo
| Autor: | Vedran Micek, Jasna Jurasović, Ivan Sabolić, Tatjana Orct, Dean Karaica |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Pathology 010501 environmental sciences Biology Kidney 01 natural sciences Biochemistry Inorganic Chemistry 03 medical and health sciences In vivo Internal medicine Blood plasma medicine Animals Rats Wistar Gender differences ICP-MS Macronutrients Micronutrients Rat organs Trace elements Inductively coupled plasma mass spectrometry 0105 earth and related environmental sciences Whole blood Sex Characteristics Brain Micronutrient Rats Trace Elements Perfusion 030104 developmental biology Endocrinology Liver Rat liver Molecular Medicine Female Digestion |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 40:104-111 |
| ISSN: | 0946-672X |
| DOI: | 10.1016/j.jtemb.2016.12.015 |
| Popis: | Concentrations of macro- and microelements in animal organs indicate the animal health status and represent reference data for animal experiments. Their levels in blood and tissues could be different between sexes, and could be different with and without blood in tissues. To test these hypotheses, in adult female and male rats the concentrations of various elements were measured in whole blood, blood plasma, and tissues from blood-containing (nonperfused) and blood-free liver, kidneys, and brain (perfused in vivo with an elements-free buffer). In these samples, 6 macroelements (Na, Mg, P, S, K, Ca) and 14 microelements (Fe, Mn, Co, Cu, Zn, Se, I, As, Cd, Hg, Pb, Li, B, Sr) were determined by inductively coupled plasma mass spectrometry following nitric acid digestion. In blood and plasma, female- or male-dominant sex differences were observed for 6 and 5 elements, respectively. In nonperfused organs, sex differences were observed for 3 (liver, brain) or 9 (kidneys) elements, whereas in perfused organs, similar differences were detected for 9 elements in the liver, 5 in the kidneys, and none in the brain. In females, perfused organs had significantly lower concentrations of 4, 5, and 2, and higher concentrations of 10, 4, and 7 elements, respectively, in the liver, kidneys, and brain. In males, perfusion caused lower concentrations of 4, 7, and 2, and higher concentrations of 1, 1, and 7 elements, respectively, in the liver, kidneys, and brain. Therefore, the residual blood in organs can significantly influence tissue concentrations of various elements and their sex-dependency. |
| Databáze: | OpenAIRE |
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