Clonal Relatedness, Phylotyping and Antimicrobial Susceptibility of Extended- spectrum-beta-lactamase Producing Uropathogenic Escherichia coli Isolates from Outpatients and Inpatients
| Autor: | Mohammad Motamedifar, Negar Azimzadeh, Abdollah Derakhshandeh, Zahra Naziri |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Male Hospitalized patients medicine.medical_treatment Antimicrobial susceptibility Microbial Sensitivity Tests medicine.disease_cause beta-Lactamases Microbiology Antibiotic resistance Intensive care Drug Resistance Multiple Bacterial Outpatients medicine Pulsed-field gel electrophoresis Prevalence Humans Uropathogenic Escherichia coli Escherichia coli Escherichia coli Infections Phylogeny Pharmacology Inpatients business.industry General Medicine Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Urinary Tract Infections Beta-lactamase Molecular Medicine Female Pfge analysis business |
| Zdroj: | Infectious disorders drug targets. 20(5) |
| ISSN: | 2212-3989 |
| Popis: | Objectives: Antibiotic resistance, phylogenetic groups and Pulsed-Field Gel Electrophoresis (PFGE) patterns were evaluated in urinary tract infection (UTI) Escherichia coli (E. coli) isolates from outpatients and inpatients. Methods: In this study, antibiotic resistance to E. coli isolated from non-hospitalized and hospitalized patients (153 outpatients and 147 inpatients ) was evaluated in Shiraz County, Iran. Phylogenetic groups and Pulse Field Gel Electrophoresis (PFGE) patterns of 143 ESBLs-producing E. coli were also assessed. Results: The prevalence of ESBL-producing E. coli was shown to be 46.4% and 49% in the outpatient and inpatient UTI E. coli isolates, respectively. Most ESBL-producers were detected on patients hospitalized in clinical surgery units (66.7%) and intensive care units (62.5%). Phylogenetic group D was the dominant group in both the outpatient and inpatient isolates (67.6% and 61.1%, respectively) and also in internal, clinical surgery and ICU units. PFGE results showed more relatedness (>80% similarity) among inpatient isolates. PFGE analysis of 49 ESBL-producing inpatient E.coli in hospital units revealed 17 different pulsotypes, consisting of 11 clones and 6 single patterns. There were no clonal patterns in outpatient isolates, and similarity among the outpatient isolates and also between inpatient and outpatient isolates was less than 80% (75% and 66%, respectively). Conclusions: The results showed extreme genomic diversity among the ESBL-producing E. coli isolates in terms of the community and multiclonal dissemination of ESBL-producing E. coli isolated from hospital units. |
| Databáze: | OpenAIRE |
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