Charge transfer dissociation of a branched glycan with alkali and alkaline earth metal adducts

Autor: David Ropartz, Hélène Rogniaux, Zachary J. Sasiene, Glen P. Jackson
Přispěvatelé: West Virginia University [Morgantown], Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRA Plateforme BIBS, Unité de Recherche Biopolymères, Interactions, Assemblages, Department of Forensic and Investigative Science [Morgantown], National Science Foundation (NSF) : CHE-1710376. United States National Institutes of Health (NIH) - USA, NIH National Institute of General Medical Sciences (NIGMS) : R01 GM114494. United States Department of Health & Human Services, National Institutes of Health (NIH) - USA:1R01GM114494-01.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Mass Spectrometry
Journal of Mass Spectrometry, Wiley-Blackwell, 2021, 56 (7), pp.e4774. ⟨10.1002/jms.4774⟩
J Mass Spectrom
ISSN: 1076-5174
1096-9888
Popis: International audience; Alkali and alkaline earth metal adducts of a branched glycan, XXXG, were analyzed with helium charge transfer dissociation (He-CTD) and low-energy collision-induced dissociation (LE-CID) to investigate if metalation would impact the type of fragments generated and the structural characterization of the analyte. The studied adducts included 1+ and 2+ precursors involving one or more of the cations: H+, Na+, K+, Ca2+, and Mg2+. Regard less of the metal adduct, He-CTD generated abundant and numerous glycosidic and cross-ring cleavages that were structurally informative and able to identify the 1,4-linkage and 1,6-branching patterns. In contrast, the LE-CID spectra mainly contained glycosidic cleavages, consecutive fragments, and numerous neutral losses, which complicated spectral interpretation. LE-CID of [M + K + H](2+) and [M + Na](+) precursors generated a few cross-ring cleavages, but they were not sufficient to identify the 1,4-linkage and 1,6-branching pattern of the XXXG xyloglucan. He-CTD predominantly generated 1+ fragments from 1+ precursors and 2+ product ions from 2+ precursors, although both LE-CID and He-CTD were able to generate 1+ product ions from 2+ adducts of magnesium and calcium. The singly charged fragments derive from the loss of H+ from the metalated product ions and the formation of a protonated complementary product ion; such observations are similar to previous reports for magnesium and calcium salts undergoing electron capture dissociation (ECD) activation. However, during He-CTD, the [M + Mg](2+) precursor generated more singly charged product ions than [M + Ca](2+), either because Mg has a higher second ionization potential than Ca or because of conformational differences and the locations of the charging adducts during fragmentation. He-CTD of the [M + 2Na](2+) and the [M + 2 K](2+) precursors generated singly charged product ions from the loss of a sodium ion and potassium ion, respectively. In summary, although the metal ions influence the mass and charge state of the observed product ions, the metal ions had a negligible effect on the types of cross-ring cleavages observed.
Databáze: OpenAIRE
Externí odkaz: