Identification of leukemia cell-derived inhibitory activity (LIA) in conditioned media from human myeloid leukemic cell line ML-2
| Autor: | V. Cukrová, J. Neuwirt, H. Kopřivová, Z. Hrkal |
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| Rok vydání: | 1986 |
| Předmět: |
Male
medicine.medical_specialty Myeloid Placenta Cell Biology Cell Line Colony-Forming Units Assay Sepharose Mice Colony-Stimulating Factors Bone Marrow Internal medicine medicine Animals Humans Macrophages Hematology General Medicine Hematopoietic Stem Cells medicine.disease Molecular biology In vitro Culture Media Hematopoiesis Ferritin Leukemia Endocrinology medicine.anatomical_structure Leukemia Myeloid Cell culture Ferritins Chromatography Gel biology.protein Isoelectric Focusing Antibody Granulocytes |
| Zdroj: | Blut. 52:51-58 |
| ISSN: | 1432-0584 0006-5242 |
| DOI: | 10.1007/bf00320142 |
| Popis: | Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5 X 10(6) ML-2 cells/ml or 1 X 10(6) ML-2 cells/ml in serum-free RPMI for 5 or 24 hours respectively, and was isolated by ultrafiltration through an XM 300 Diaflo membrane followed by chromatography on Sepharose 6 B. Ferritin, prepared from human placenta, had the same inhibitory effect on CFU-GM. Antibodies against human placental ferritin completely inactivated the inhibitory effect of both human placental ferritin and the factor released from ML-2 cells. The inhibitory activity produced by the cell-line ML-2 was considered as LIA (leukemia cell-derived inhibitory activity) earlier found in HL-60 cell line and AML and CML cells. |
| Databáze: | OpenAIRE |
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