Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover
| Autor: | Dahai Gao, Bruce E. Dale, Venkatesh Balan, Chandraraj Krishnan, Shishir P. S. Chundawat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Glycosyl hydrolases
Proteomics Molecular biology xylan endo 1 3 beta xylosidase enzyme purification ammonia xylan 1 4 beta xylosidase Pichia Mass loadings chemistry.chemical_compound lignocellulose Bioreactors Pichia pastoris Hemicellulase Cellulases Food science Waste Management and Disposal fermentation N-Glycosyl Hydrolases chemistry.chemical_classification biology Catalysts Beta-glucosidase Hydrolysis General Medicine Fermentable sugars cellulose Enzymes corn Corn stover Biochemistry priority journal sugar Cellobiohydrolases Microplates glycosidase Optimal composition Optimization Environmental Engineering Bioengineering High-throughput Cellulase Family 11 Xylosidase Saccharification enzyme isolation Zea mays Synergistic interaction Aspergillus nidulans Cellobiohydrolase I Total protein Endoglucanase I Endoxylanase process optimization Enzymatic hydrolysis Enzymatic Activity controlled study Cellulose cellulose 1 4 beta cellobiosidase Glucan nonhuman Ethanol beta glucosidase Renewable Energy Sustainability and the Environment Four-core Plant Components Aerial AFEX pretreatment glucan synthase Ammonia fibers enzyme Planning chemistry Biofuels Strategic planning biology.protein Sugar (sucrose) Hemicellulases Glucosidase Cellulosic ethanol protein Ligno-cellulosics |
| Zdroj: | IndraStra Global. |
| ISSN: | 2381-3652 |
| Popis: | In this work, six core glycosyl hydrolases (GH) were isolated and purified from various sources to help rationally optimize an enzyme cocktail to digest ammonia fiber expansion (AFEX) treated corn stover. The four core cellulases were endoglucanase I (EG I, GH family 7B), cellobiohydrolase I (CBH I, GH family 7A), cellobiohydrolase II (CBH II, GH family 6A) and ?-glucosidase (?G, GH family 3). The two core hemicellulases were an endo-xylanase (EX, GH family 11) and a ?-xylosidase (?X, GH family 3). Enzyme family and purity were confirmed by proteomics. Synergistic interactions among the six core enzymes for varying relative and total protein loading (8.25, 16.5 and 33 mg/g glucan) during hydrolysis of AFEX-treated corn stover was studied using a high-throughput microplate based protocol. The optimal composition (based on% protein mass loading) of the cocktail mixture was CBH I (28.4%): CBH II (18.0%): EG I (31.0%): EX (14.1%): ?G (4.7%): ?X (3.8%). These results demonstrate a rational strategy for the development of a minimal, synergistic enzymes cocktail that could reduce enzyme usage and maximize the fermentable sugar yields from pretreated lignocellulosics. � 2009 Elsevier Ltd. |
| Databáze: | OpenAIRE |
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