Route of tracer administration does not affect ileal endogenous nitrogen recovery measured with the 15N-Isotope dilution technique in pigs fed rapidly digestible diets
| Autor: | Marianne van 't End, Huug Boer, Seerp Tamminga, G. Henk Visser, M.W.A. Verstegen, Berthe Verstappen, C A Carina Steendam, William R Caine |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Animal Nutrition Nitrogen Swine Anacardiaceae Medicine (miscellaneous) Indicator Dilution Techniques Isotope dilution Excretion Animal science Oral administration Suidae Ileum Casein Internal medicine ileal digesta medicine Animals Dry matter amino-acid flows infusion protein-free Nutrition and Dietetics biology Nitrogen Isotopes Chemistry Caseins Starch biology.organism_classification Diervoeding kg liveweight pig losses Diet growing pigs Postprandial Endocrinology WIAS distal ileum Digestion excretion leucine Tannins |
| Zdroj: | The Journal of Nutrition 134 (2004) 11 The Journal of Nutrition, 134(11), 3068-3075 |
| ISSN: | 0022-3166 |
| Popis: | The (15)N-isotope dilution technique ((15)N-IDT), with either pulse-dose oral administration or continuous i.v. administration of [(15)N]-l-leucine (carotid artery), both at 5 mg/(kg body weight . d), was used to measure ileal (postvalve T-cecum cannula) endogenous nitrogen recovery (ENR) in pigs (9 +/- 0.6 kg). Diets were cornstarch, enzyme-hydrolyzed casein with no (control) or high (4%) content of quebracho extract (Schinopsis spp.) rich in condensed tannins. Blood was sampled from a catheter in the external jugular vein. Mean plasma (15)N-enrichment at d 8-10 was higher (P = 0.0009) after i.v. than after oral administration [0.0356 vs. 0.0379 atom% excess (APE)]. Plasma (15)N-enrichment for i.v. infused pigs was 0.01117 APE higher (P < 0.0001) and for orally dosed pigs 0.0081 APE lower (P < 0.0001) at 11 h postprandial compared with 1 h postprandial. Apparent ileal N digestibility was higher (P < 0.0001) for the control (85.5%) than for the quebracho diet (69.5%). ENR was calculated from the ratio of (15)N-enrichment of plasma and digesta. The ENR for the quebracho diet was approximately 300% higher than for the control diet (6.03 vs. 1.94 g/kg dry matter intake, P < 0.001). The real N digestibility (92.2 +/- 0.4%) was equal for both diets (P = 0.1030) and both tracer methods (P = 0.9730). We concluded that oral administration of [(15)N]leucine provides reasonable estimates of ENR in pigs fed semipurified diets with high or low content of tannins; however, one must be careful in extrapolating this conclusion to studies with other protein sources or feeding frequencies. |
| Databáze: | OpenAIRE |
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