Cloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution

Autor: Pascal Hoarau, Michèle Roméo, M. Gnassia-Barelli, Maria João Bebianno, Gautier Damiens
Rok vydání: 2006
Předmět:
Enzymologic
Gills
Gill
Transcription
Genetic
Physiology
Health
Toxicology and Mutagenesis
Messenger
Toxicology
Biochemistry
Transcription (biology)
Gene expression
Cloning
Molecular
Phylogeny
Gene expression regulation
biology
Environmental monitoring
General Medicine
Mytilus
Transcription
Cadmium
Environmental Monitoring
animal structures
Molecular Sequence Data
Glutathione S-transferase pi
Chemical
Gene Expression Regulation
Enzymologic
Water pollutants
Genetic
Molecular sequence data
Complementary DNA
Benzo(a)pyrene
Animals
RNA
Messenger
Northern blot
Gene
Portugal
Environmental Exposure
Cell Biology
Mussel
Environmental exposure
biology.organism_classification
Molecular biology
Digestive system
Glutathione S-Transferase pi
RNA
Digestive System
Biomarkers
Water Pollutants
Chemical
Cloning
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 1532-0456
DOI: 10.1016/j.cbpc.2006.02.007
Popis: Glutathione S-transferase is involved in the detoxication of many chemical compounds. Northern blot analysis of mRNA GST gene from the mussel Mytilus galloprovincialis shows the presence of a transcript of 850 bp (GenBank accession no. Gi:22094808 and AF527010). The cDNA cloned sequence is constituted by an ORF of 621 bp encoding for a protein of 23,700 Da present in the gills and digestive gland of M galloprovincialis. The sequence, called Mg (M. galloprovincialis) GST-p, is clearly related to the pi class GST. M. galloprovincialis treated with Cd (200 microg/L) and BaP (100 microg/L) or co-treated with Cd and BaP take up cadmium in the gills (16.2 +/- 4.2 and 12.6 +/- 1.2 microg Cd/g dry wt. after exposure to Cd and Cd + BaP, respectively) and in the digestive gland. The transcription of GST-pi gene, by semi-quantitative RT-PCR, shows the lowest value in the digestive glands of mussels exposed to BaP, whereas the treatment with cadmium and the co-treatment with cadmium and BaP evoke GST-pi gene expression higher than controls. Mussels collected from six sites along the south coast of Portugal show different GST-pi transcription levels, some of which are related to their pollutant content. Portuguese Foundation for Science and Technology info:eu-repo/semantics/publishedVersion
Databáze: OpenAIRE
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