Evidence for the synthesis and release of strongly immunosuppressive, noncytotoxic substances by Streptococcus intermedius
| Autor: | M. T. Porto, T B Higerd, C B Loadholt, Jean-Michel Goust, H. Hugh Fudenberg, J Munoz, M.P. Arala-Chaves |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Leukocyte migration
biology Streptococcus Hemolytic Plaque Technique General Medicine Streptococcus intermedius Cytotoxicity Tests Immunologic Lymphocyte Activation biology.organism_classification In vitro Microbiology Mice Inbred C57BL Mice Immune system Antigen In vivo Extracellular Animals Humans Lymphocytes Immunosuppressive Agents Research Article |
| Zdroj: | Journal of Clinical Investigation. 64:871-883 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci109553 |
| Popis: | A B S T RA C T Products secreted by Streptococcus intermedius were studied for their effects on the immune response. Three different preparations of crude extracellular products from S. intermedius (CEP-Si) were found to have powerful suppressor activity in vitro as shown by inhibition of human lymphocyte proliferation (uptake of [3H]thymidine) and protein synthesis in response to a wide variety of stimulants, including mitogens and antigens, and suppression of plaque formation by human cells in response to sheep erythrocytes. CEP-Si was noncytotoxic, because cells incubated with high concentrations of CEP-Si and subsequently washed were viable and recovered their ability to respond to mitogens, and because leukocyte migration was not inhibited by CEP-Si, nor was the release of leukocyte migration inhibitory factor from sensitized lymphocytes. The possibility of antigen or mitogen competition was excluded. The effects of CEP-Si in vitro were time dependent and did not require the presence of monocytes. Cells pretreated with CEP-Si and then washed suppressed plaque formation by fresh autologous cells in highly stimulated cultures. CEP-Si injected into C57BL/6 mice also strongly suppressed their immune response to sheep erythrocytes, and the in vivo suppression was correlated with the effects of CEP-Si in vitro. |
| Databáze: | OpenAIRE |
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