A nanosecond pulsed electric field (nsPEF) can affect membrane permeabilization and cellular viability in a 3D spheroids tumor model
| Autor: | Marie-Pierre Rols, Jelena Kolosnjaj-Tabi, Rosa Orlacchio, Lynn Carr, Muriel Golzio, Geraldine Alberola, Philippe Lévêque, Delia Arnaud-Cormos |
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| Přispěvatelé: | XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, BIO-INGENIERIE (XLIM-BIO-INGENIERIE), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), RF-ELITE : RF-Electronique Imprimée pour les Télécommunications et l'Energie (XLIM-RFEI), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), European Project: 207249, H2020-EU.1.2.1. - FET Open,737164,SUMCASTEC(2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cell Membrane Permeability
[SDV.BIO]Life Sciences [q-bio]/Biotechnology Cell Survival Nanosecond pulsed electric field (nsPEF) Biophysics 02 engineering and technology 01 natural sciences 3D cell culture Electricity In vivo Neoplasms Spheroids Cellular Electric field Electrochemistry Humans Physical and Theoretical Chemistry Electropulsation ComputingMilieux_MISCELLANEOUS Membrane permeabilization Chemistry Electroporation Bipolar cancellation 010401 analytical chemistry Spheroid General Medicine Nanosecond HCT116 Cells 021001 nanoscience & nanotechnology 0104 chemical sciences [SPI.ELEC]Engineering Sciences [physics]/Electromagnetism Interphase Spheroids 0210 nano-technology |
| Zdroj: | Bioelectrochemistry Bioelectrochemistry, Elsevier, 2021, 141, pp.107839. ⟨10.1016/j.bioelechem.2021.107839⟩ Bioelectrochemistry, 2021, 141, pp.107839. ⟨10.1016/j.bioelechem.2021.107839⟩ |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2021.107839⟩ |
| Popis: | International audience; Three-dimensional (3D) cellular models represent more realistically the complexity of in vivo tumors compared to 2D cultures. While 3D models were largely used in classical electroporation, the effects of nanosecond pulsed electric field (nsPEF) have been poorly investigated. In this study, we evaluated the biological effects induced by nsPEF on spheroid tumor model derived from the HCT-116 human colorectal carcinoma cell line. By varying the number of pulses (from 1 to 500) and the polarity (unipolar and bipolar), the response of nsPEF exposure (10 ns duration, 50 kV/cm) was assessed either immediately after the application of the pulses or over a period lasting up to 6 days. Membrane permeabilization and cellular death occurred following the application of at least 100 pulses. The extent of the response increased with the number of pulses, with a significant decrease of viability, 24 h post-exposure, when 250 and 500 pulses were applied. The effects were highly reduced when an equivalent number of bipolar pulses were delivered. This reduction was eliminated when a 100 ns interphase interval was introduced into the bipolar pulses. Altogether, our results show that nsPEF effects, previously observed at the single cell level, also occur in more realistic 3D tumor spheroids models. |
| Databáze: | OpenAIRE |
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