Determination of the amino acid sequence of apamin by tandem mass spectrometry
| Autor: | N. Ordsmith, Albert Marcual, Catherine Lange, R. S. Bordoli, Laure Marvin |
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| Rok vydání: | 1994 |
| Předmět: |
Chromatography
Molecular mass Protein mass spectrometry Hydrolysis Organic Chemistry Molecular Sequence Data Peptide sequence tag Apamin Tandem mass tag Tandem mass spectrometry Mass spectrometry Sample preparation in mass spectrometry Mass Spectrometry Analytical Chemistry Molecular Weight chemistry.chemical_compound Dithiothreitol chemistry Trypsin Amino Acid Sequence Spectroscopy |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 8(7) |
| ISSN: | 0951-4198 |
| Popis: | A new strategy has been developed for the determination of the amino acid sequence of apamin, a two disulfide-bond neurotoxic oligopeptide. Relative molecular mass up to about 2000 was determined using electrospray mass spectrometry. This technique shows that three basic amino acid residues are present in apamin. A hydrophobic tryptic digest fragment was sequenced using fast-atom bombardment combined with tandem mass spectrometry. But the full sequence has been directly observed using a tandem mass spectrometer: the daughter-ion mass spectrum of protonated molecules of dithiothreitol-reduced apamin gives the sequence. |
| Databáze: | OpenAIRE |
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