Interstitial water microbial communities as an indicator of microbial denitrifying capacity in wood-chip bioreactors
| Autor: | Mark Button, Bruce C. Anderson, Sonja Bissegger, Lloyd Rozema, Soheil Fatehi-Pouladi, Kela P. Weber, Brent Wootton |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
Denitrification 010504 meteorology & atmospheric sciences Wastewater 010501 environmental sciences Waste Disposal Fluid complex mixtures 01 natural sciences Water Purification Carbon utilization chemistry.chemical_compound Denitrifying bacteria Bioreactors Nitrate Bioreactor Environmental Chemistry Waste Management and Disposal Effluent 0105 earth and related environmental sciences 2. Zero hunger Nitrates biology Microbiota technology industry and agriculture equipment and supplies Pulp and paper industry biology.organism_classification Wood Pollution 6. Clean water Biodegradation Environmental chemistry Microbial population biology Environmental science Oxidation-Reduction Typha angustifolia |
| Zdroj: | Science of The Total Environment. 655:720-729 |
| ISSN: | 0048-9697 |
| Popis: | The discharge from food production greenhouses (greenhouse effluent) contains high nutrient and salt concentrations, which, if directly released, can have adverse effects on the environment. Wood-chip bioreactors are increasingly popular passive water treatment systems favoured for their economical denitrification in treating agricultural field tile drainage. Microbial communities are central to denitrification; however little is known about the maturation of microbial communities in wood-chip bioreactors treating greenhouse effluents. In this study, multiple subsurface flow wood-chip bioreactors, each vegetated with a different plant species, together with an unplanted unit, received synthetic greenhouse effluent with elevated nitrate concentrations. The hybrid bioreactors were operated for over 2 years, during which time water samples were collected from the inlet, outlet and within the reactors. The increasing denitrification rate in the bioreactor planted with Typha angustifolia (narrowleaf cattail) correlated with increasing microbial activity and metabolic richness, measured by the carbon utilization patterns in Biolog® EcoPlates. Increased denitrifying gene (nirS) copies (determined by quantitative polymerase chain reaction, qPCR), and near-complete nitrate removal were observed in the T. angustifolia and unplanted reactors after 16 and 23 months of operation respectively. The findings suggested that an acclimation period of at least one year can be expected in unseeded bioreactors planted with T. angustifolia, while bioreactors without vegetation may require a longer time to maximize their denitrification capacity. These results are important for the design and operation of wood-chip bioreactors, which are expected to be more commonly applied in the future. |
| Databáze: | OpenAIRE |
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