Differential Modulation of Angiogenesis by Erythropoiesis-Stimulating Agents in a Mouse Model of Ischaemic Retinopathy
| Autor: | Pauline M. Rudd, Reinhold J. Medina, Carmel M. McVicar, Alan W. Stitt, Claire L. Kitson, Ross Hamilton, Jodie L. Abrahams, Tom A. Gardiner, Dash Durga, Liza Colhoun |
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| Rok vydání: | 2010 |
| Předmět: |
Darbepoetin alfa
Angiogenesis Science Biology Retina Neovascularization Mice Ophthalmology/Diabetic Retinopathy Vasculogenesis Microscopy Electron Transmission Retinal Diseases Ischemia In vivo hemic and lymphatic diseases medicine Animals Erythropoietin Cells Cultured Chromatography High Pressure Liquid Epoetin beta Multidisciplinary Neovascularization Pathologic Reverse Transcriptase Polymerase Chain Reaction Cell Biology Recombinant Proteins Mice Inbred C57BL Disease Models Animal Diabetes and Endocrinology Immunology Hematinics Cancer research Medicine Erythropoiesis medicine.symptom Research Article medicine.drug |
| Zdroj: | PLoS ONE, Vol 5, Iss 7, p e11870 (2010) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0011870 |
| Popis: | BackgroundErythropoiesis stimulating agents (ESAs) are widely used to treat anaemia but concerns exist about their potential to promote pathological angiogenesis in some clinical scenarios. In the current study we have assessed the angiogenic potential of three ESAs; epoetin delta, darbepoetin alfa and epoetin beta using in vitro and in vivo models.Methodology/principal findingsThe epoetins induced angiogenesis in human microvascular endothelial cells at high doses, although darbepoetin alfa was pro-angiogenic at low-doses (1-20 IU/ml). ESA-induced angiogenesis was VEGF-mediated. In a mouse model of ischaemia-induced retinopathy, all ESAs induced generation of reticulocytes but only epoetin beta exacerbated pathological (pre-retinal) neovascularisation in comparison to controls (pConclusionsThis study has implications for treatment of patients as there are clear differences in the angiogenic potential of the different ESAs. |
| Databáze: | OpenAIRE |
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