Interaction between sulfogalactosylceramide and dimyristoylphosphatidylcholine increases the orientational fluctuation of their lipid hydrocarbon chains
| Autor: | Nongnuj Tanphaichitr, Pascale Jaklis, Morris Kates, Patrick T. T. Wong, Danielle Julie Carrier, Mayssa Attar |
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| Rok vydání: | 1998 |
| Předmět: |
inorganic chemicals
Infrared spectroscopy Galactosylceramides Biochemistry Thermotropic crystal chemistry.chemical_compound Phosphatidylcholine Spectroscopy Fourier Transform Infrared Animals Organic chemistry Molecule heterocyclic compounds Methylene Fourier transform infrared spectroscopy Molecular Biology Brain Chemistry Liposome Sulfoglycosphingolipids Hydrogen bond Organic Chemistry technology industry and agriculture Hydrogen Bonding Cell Biology Hydrocarbons Crystallography chemistry Liposomes cardiovascular system Cattle lipids (amino acids peptides and proteins) Dimyristoylphosphatidylcholine |
| Zdroj: | Chemistry and Physics of Lipids. 94:227-238 |
| ISSN: | 0009-3084 |
| Popis: | The objective of this study was to investigate the interaction between sulfogalactosylceramide (SGC) and dimyristoylphosphatidylcholine (DMPC) in a mixed model liposomal system (molar ratio SGC:DMPC, 2:3). Structural and dynamic changes of the liposome components were monitored by Fourier-transform infrared spectroscopy (FTIR). Thermotropic FTIR analysis of the mixed liposomes showed a single gel/liquid crystalline phase transition, centered at approximately 42 degrees C. Spectral changes of the amide and ester C = O bands arising from functional groups at the interfacial region indicated a reduced hydrogen bonding of these groups in the mixed liposomes. Pressure-tuning FTIR of mixed liposomes showed that the methylene chains of SGC and DMPC were more orientationally disordered than those of the individual lipid SGC liposomes or DMPC liposomes. These results suggest that the mixed liposomes (molar ratio SGC:DMPC, 2:3) consisted of a homogeneous mixture of SGC and DMPC molecules in which mutual shielding reduced hydrogen bonding in the interfacial region, with a concurrent increase in the orientational disorder of the hydrocarbon chains of both SGC and DMPC. |
| Databáze: | OpenAIRE |
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