Structure and Mutation of the Native Amine Dehydrogenase MATOUAmDH2

Autor:	Megan Bennett, Laurine Ducrot, Carine Vergne‐Vaxelaire, Gideon Grogan
Rok vydání:	2022
Předmět:	Organic Chemistry Mutation Biocatalysis Molecular Medicine Amines NAD Oxidoreductases Molecular Biology Biochemistry NADP Amination Substrate Specificity
Zdroj:	Chembiochem : a European journal of chemical biology. 23(10)
ISSN:	1439-7633
Popis:	Native amine dehydrogenases (nat-AmDHs) have recently emerged as a potentially valuable new reservoir of enzymes for the sustainable and selective synthesis of chiral amines, catalyzing the NAD(P)H-dependent ammoniation of carbonyl compounds with high activity and selectivity. MATOUAmDH2, recently identified from the Marine Atlas of Tara Oceans Unigenes (MATOUv1) database of eukaryotic genes, displays exceptional catalytic performance against its best identified substrate, isobutyraldehyde, as well as having broader substrate scope than other nat-AmDHs. In the interests of providing a platform for the rational engineering of this and other nat-AmDHs, we have determined the structure of MATOUAmDH2 in complex with NADP
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2600eae2e32384dd8c0376ba920e8dc3 https://pubmed.ncbi.nlm.nih.gov/35349204 Zobrazit plný text záznamu Plný text