Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review
| Autor: | Xiaoyan Ma, Ning Wang, Yi-Xin Huo, Said Nawab |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
business.industry
Fossil fuel Biofuel production lcsh:QR1-502 Bioengineering Review Applied Microbiology and Biotechnology lcsh:Microbiology Renewable energy Synthetic biology 1-Butanol Bioenergy Biofuel Non-native hosts Sustainability Synthetic pathways Production (economics) Business Biochemical engineering Genetic Engineering Biotechnology Renewable resource |
| Zdroj: | Microbial Cell Factories, Vol 19, Iss 1, Pp 1-16 (2020) Microbial Cell Factories |
| ISSN: | 1475-2859 |
| DOI: | 10.1186/s12934-020-01337-w |
| Popis: | Background Owing to the increase in energy consumption, fossil fuel resources are gradually depleting which has led to the growing environmental concerns; therefore, scientists are being urged to produce sustainable and ecofriendly fuels. Thus, there is a growing interest in the generation of biofuels from renewable energy resources using microbial fermentation. Main text Butanol is a promising biofuel that can substitute for gasoline; unfortunately, natural microorganisms pose challenges for the economical production of 1-butanol at an industrial scale. The availability of genetic and molecular tools to engineer existing native pathways or create synthetic pathways have made non-native hosts a good choice for the production of 1-butanol from renewable resources. Non-native hosts have several distinct advantages, including using of cost-efficient feedstock, solvent tolerant and reduction of contamination risk. Therefore, engineering non-native hosts to produce biofuels is a promising approach towards achieving sustainability. This paper reviews the currently employed strategies and synthetic biology approaches used to produce 1-butanol in non-native hosts over the past few years. In addition, current challenges faced in using non-native hosts and the possible solutions that can help improve 1-butanol production are also discussed. Conclusion Non-native organisms have the potential to realize commercial production of 1- butanol from renewable resources. Future research should focus on substrate utilization, cofactor imbalance, and promoter selection to boost 1-butanol production in non-native hosts. Moreover, the application of robust genetic engineering approaches is required for metabolic engineering of microorganisms to make them industrially feasible for 1-butanol production. |
| Databáze: | OpenAIRE |
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