A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids
| Autor: | Sin-Cih Sun, Wan-Yu Chung, Ola Kamar, Dmitry M. Kolpashchikov, Chieh-Hua Lin, Yu-Chieh Liao, Min-Shi Lee, Min-Chieh Chuang |
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| Rok vydání: | 2017 |
| Předmět: |
Deoxyribozyme
Biology 010402 general chemistry medicine.disease_cause 01 natural sciences Genome Catalysis Article Complementary DNA Materials Chemistry medicine Nucleotide Fluorescent Dyes Genetics chemistry.chemical_classification Mutation 010405 organic chemistry Point mutation Optical Imaging Metals and Alloys RNA General Chemistry DNA Catalytic Molecular biology 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Enterovirus A Human chemistry DNA Viral Ceramics and Composites Nucleic acid |
| Zdroj: | Chemical communications (Cambridge, England). 53(76) |
| ISSN: | 1364-548X |
| Popis: | Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases. |
| Databáze: | OpenAIRE |
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