High glucose-6-phosphatase activity in non-pigmented epithelial cells of rabbit ciliary body
| Autor: | Jun Watanabe, Youko Asaka, Kazuo Kanai, Shinsuke Kanamura |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Pars plana medicine.medical_specialty Histology Golgi Apparatus Biology Endoplasmic Reticulum Aqueous Humor chemistry.chemical_compound Ciliary processes Ciliary body Hexokinase Internal medicine medicine Animals Glycogen Histocytochemistry Endoplasmic reticulum Ciliary Body Endothelium Corneal Molecular biology Staining Microscopy Electron Glucose medicine.anatomical_structure Endocrinology chemistry Glucose-6-Phosphatase Pars plicata Rabbits Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 39:1113-1120 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/39.8.1649856 |
| Popis: | For study of the origin of glucose in the aqueous humor, glucose-6-phosphatase (G6Pase) and hexokinase activities, and glycogen, were cytochemically examined in the ciliary body (CB) of rabbit. G6Pase activity was also assayed biochemically. The staining reaction for G6Pase activity was strong in the non-pigmented epithelium (NPE) in the pars plana and tips of ciliary processes in the region containing large ciliary pockets within the pars plicata. NPE cells contained abundant reaction product for G6Pase activity in the endoplasmic reticulum (ER) and nuclear envelope. However, NPE in other regions of the CB and pigmented epithelium (PE) of CB, and other areas surrounding the anterior and (PE) of CB, and other areas surrounding the anterior and posterior chambers, showed weak or no G6Pase staining reaction. Biochemical G6Pase activity in the whole ciliary body was relatively high. Both NPE and PE in the pars plana and the tips showed strong staining reaction for hexokinase activity but no staining for glycogen. Furthermore, NPE cells in the tips bore large aggregates of smooth ER and many Golgi apparati. These suggest that the high G6Pase activity in NPE cells in the pars plana and the tips is related to glucose release into the aqueous humor. |
| Databáze: | OpenAIRE |
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