DNA template strand segregation in developing zebrafish
| Autor: | Nathan W. Luedtke, Stella M. K. Glasauer, Anne B. Neef, Stephan C.F. Neuhauss, Therese Triemer |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Cell division biology Clinical Biochemistry DNA biology.organism_classification Biochemistry Immortal DNA strand hypothesis Cell biology chemistry.chemical_compound chemistry Coding strand Drug Discovery Molecular Medicine Sister chromatids Animals Stem cell Molecular Biology Zebrafish Bromodeoxyuridine |
| Zdroj: | Cell chemical biology. 28(11) |
| ISSN: | 2451-9448 |
| Popis: | Asymmetric inheritance of sister chromatids has long been predicted to be linked to discordant fates of daughter cells and even hypothesized to minimize accumulation of mutations in stem cells. Here, we use (2'S)-2'-deoxy-2'-fluoro-5-ethynyluridine (F-ara-EdU), bromodeoxyuridine (BrdU), and light sheet microscopy to track embryonic DNA in whole zebrafish. Larval development results in rapid depletion of older DNA template strands from stem cell niches in the retina, brain, and intestine. Prolonged label retention occurs in quiescent progenitors that resume replication in later development. High-resolution microscopy reveals no evidence of asymmetric template strand segregation in >100 daughter cell pairs, making it improbable that asymmetric DNA segregation prevents mutational burden according to the immortal strand hypothesis in developing zebrafish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|