Deletion of Krüppel-like factor-4 promotes axonal regeneration in mammals
| Autor: | Xu-Zhen Qin, Hao-Nan Zhang, Hong-Cheng Zhang, Xin-Ya Fu, Shi-Bin Qi, Ji-Le Xie, Jin-Jin Ma, Yan-Xia Ma, Jin-Hui Xu, Saijilafu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Nervous system corticospinal tract dorsal root ganglion medicine.medical_treatment spinal cord crushl Biology Retinal ganglion lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Dorsal root ganglion stomatognathic system peripheral nervous system medicine axonal regeneration klf4 sciatic nerve crush sensorimotor cortex lcsh:Neurology. Diseases of the nervous system Regeneration (biology) fungi Sciatic nerve injury medicine.disease Klf4 Cell biology 030104 developmental biology medicine.anatomical_structure nervous system Peripheral nervous system embryonic structures Sciatic nerve sense organs Axotomy 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research, Vol 16, Iss 1, Pp 166-171 (2021) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | Axonal regeneration plays an important role in functional recovery after nervous system damage. However, after axonal injury in mammals, regeneration is often poor. The deletion of Kruppel-like factor-4 (Klf4) has been shown to promote axonal regeneration in retinal ganglion cells. However, the effects of Klf4 deletion on the corticospinal tract and peripheral nervous system are unknown. In this study, using a mouse model of sciatic nerve injury, we show that the expression of Klf4 in dorsal root ganglion sensory neurons was significantly reduced after peripheral axotomy, suggesting that the regeneration of the sciatic nerve is associated with Klf4. In vitro, dorsal root ganglion sensory neurons with Klf4 knockout exhibited significantly enhanced axonal regeneration. Furthermore, the regeneration of the sciatic nerve was enhanced in vivo following Klf4 knockout. Finally, AAV-Cre virus was used to knockout the Klf4 gene in the cortex. The deletion of Klf4 enhanced regeneration of the corticospinal tract in mice with spinal cord injury. Together, our findings suggest that regulating KLF4 activity in neurons is a potential strategy for promoting axonal regeneration and functional recovery after nervous system injury. This study was approved by the Animal Ethics Committee at Soochow University, China (approval No. SUDA20200316A01). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|