miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma
| Autor: | Christos K. Kontos, Stamatia-Maria Rapti, Panagiotis G. Adamopoulos, Iordanis N. Papadopoulos, Andreas Scorilas |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Oncology Cancer Research medicine.medical_specialty Colorectal cancer Kaplan-Meier Estimate Adenocarcinoma Recurrence Internal medicine microRNA medicine Humans Pathological Aged Aged 80 and over Oncogene business.industry Cancer Middle Aged Cell cycle Prognosis medicine.disease Molecular medicine Gene Expression Regulation Neoplastic MicroRNAs Real-time polymerase chain reaction Lymphatic Metastasis Female Neoplasm Recurrence Local Colorectal Neoplasms business |
| Zdroj: | International Journal of Oncology. 46:849-859 |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2014.2775 |
| Popis: | Colorectal adenocarcinoma constitutes the most frequent form of colorectal cancer and a serious cause of cancer-related deaths. The expression of multiple miRNAs, including miR-224, is deregulated in colorectal adenocar- cinoma. The aim of this study was the investigation of the prognostic value of miR-224 in colorectal adenocarcinoma. For this purpose, total RNA was isolated from 115 colorectal adenocarcinomas and 66 adjacent non-cancer mucosae. Total RNA (2 µg) was polyadenylated and reverse transcribed. A quantitative PCR method based on SYBR-Green chemistry was developed and applied for the quantification of miR-224 levels, followed by extensive biostatistical analysis. miR-224 levels in malignant colorectal adenocarcinomas ranged between 1.81 and 187.75 RQU (miR-224 copies/1,000 SNORD48 copies) with a median of 34.27, and were significantly elevated, compared to miR-224 levels in adjacent non-cancer mucosae (p |
| Databáze: | OpenAIRE |
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