Bronchial provocation with cat allergen: correlation between the individual IgE-CRIE pattern and the occurrence of a late allergic reaction
| Autor: | Vincent Aubert, JG Frey, Ph. Leuenberger, Alain Pécoud, B. Mosimann |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Allergy Adolescent Immunology Provocation test Immunoelectrophoresis Immunoglobulin E medicine.disease_cause Bronchial Provocation Tests Allergen Albumins Forced Expiratory Volume medicine Immunology and Allergy Humans Hypersensitivity Delayed Glycoproteins biology medicine.diagnostic_test business.industry Allergens medicine.disease Asthma Delayed hypersensitivity Allergic response biology.protein Female Antibody business |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 24(1) |
| ISSN: | 0954-7894 |
| Popis: | Summary Twenty-one mild asthmatic patients allergic to cat dander underwent a bronchial provocation test (BPT) with a cat extract. An early allergic response (EAR) was observed in all 21 patients and a late allergic response (LAR) in 8/21 patients. In the EAR, the patients with subsequent LAR had a greater full in FEV1, Their baseline FEV1, and total dose of inhaled allergen were not significantly different from patients who did not develop a LAR, but their serum specific IgE level was higher. Crossed immunoelectrophoresis (CIE) of the same cal extract showed that it contained eight different proteins. An IgE-CRIE was obtained from all 21 patients, using radiolabelled anti-lgE and autoradiography. Radiolabelled standards allowed a semiquantitative scoring of the radiosiaining. The CRIE pattern of the eight patients with LAR showed a higher score of radiostaining and a greater number of proteins hound to IgE. The two major allergen cal albumin and Fel d I bound equally to IgE of patients with and without LAR whereas another protein (antigen No. 7) bound to IgE of 100% of patients with LAR but of only 38% of patients without LAR. These data suggest that the pattern of the IgE response to specific proteins of a cat extract may be related to the occurrence of LAR after BPT with this allergen. |
| Databáze: | OpenAIRE |
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