Sugar exchanges in arbuscular mycorrhiza: RiMST5 and RiMST6, two novel Rhizophagus irregularis monosaccharide transporters, are involved in both sugar uptake from the soil and from the plant partner

Autor: Christine Arnould, Nassima Ait Lahmidi, Odile Chatagnier, Daniel Wipf, Laurent Bonneau, Pierre-Emmanuel Courty, Daphnée Brulé, Joan Doidy, Guido Lingua, Graziella Berta
Přispěvatelé: Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Department of Environmental Sciences, Botany, Zurich Basel Plant Science Center, University of Basel (Unibas)-Universität Zürich [Zürich] = University of Zurich (UZH)-University of Basel (Unibas)-Universität Zürich [Zürich] = University of Zurich (UZH), Amedeo Avogadro University of Eastern Piedmont
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Rhizophagus irregularis
Light
Physiology
[SDV]Life Sciences [q-bio]
Plant Science
arbuscular mycorrhizal fungus
01 natural sciences
rhizophagus irregularis
Glomeromycota
Soil
Gene Expression Regulation
Plant
Mycorrhizae
Medicago
Phylogeny
2. Zero hunger
Mutualism (biology)
Fungal protein
Reverse Transcriptase Polymerase Chain Reaction
glucose specific
Monosaccharides
food and beverages
high affinity H+ co-transporter
high affinity transporter
Arbuscular mycorrhiza
Biochemistry
[SDE]Environmental Sciences
Fungus
Saccharomyces cerevisiae
Biology
Fungal Proteins
03 medical and health sciences
Symbiosis
Stress
Physiological
Botany
Genetics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
RNA
Messenger
Obligate
Cell Membrane
Genetic Complementation Test
fungi
MST5
MST6
Membrane Transport Proteins
15. Life on land
monosaccharide transporter
biology.organism_classification
030104 developmental biology
Glucose
010606 plant biology & botany
Zdroj: Plant Physiology and Biochemistry
Plant Physiology and Biochemistry, Elsevier, 2016, 107, pp.354-363. ⟨10.1016/j.plaphy.2016.06.023⟩
ISSN: 0981-9428
Popis: SPE IPM INRA UB CT1; International audience; Arbuscular mycorrhizal (AM) fungi are associated with about 80% of land plants. AM fungi provide inorganic nutrients to plants and in return up to 20% of the plant-fixed CO2 is transferred to the fungal symbionts. Since AM fungi are obligate biotrophs, unraveling how sugars are provided to the fungus partner is a key for understanding the functioning of the symbiosis. In this study, we identified two new monosaccharide transporters from Rhizophagus irregularis (RiMST5 and RiMST6) that we characterized as functional high affinity monosaccharide transporters. RiMST6 was characterized as a glucose specific, high affinity H(+) co-transporter. We provide experimental support for a primary role of both RiMST5 and RiMST6 in sugar uptake directly from the soil. The expression patterns of RiMSTs in response to partial light deprivation and to interaction with different host plants were investigated. Expression of genes coding for RiMSTs was transiently enhanced after 48h of shading and was unambiguously dependent on the host plant species. These results cast doubt on the 'fair trade' principle under carbon-limiting conditions. Therefore, in light of these findings, the possible mechanisms involved in the modulation between mutualism and parasitism in plant-AM fungus interactions are discussed. Copyright 2016 Elsevier Masson SAS. All rights reserved.
Databáze: OpenAIRE
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