Three-Dimensional BioAssembly Tool for Generating Viable Tissue-Engineered Constructs
Autor: | Robert Parkhill, Alice L. Stone, Mark W. Simpkins, Cynthia M. Smith, Robert L. Stewart, William L. Warren, Stuart K. Williams, Anatoly Kachurin |
---|---|
Rok vydání: | 2004 |
Předmět: |
Collagen type
Materials science Tissue engineered Tissue Engineering Cell Culture Techniques General Engineering Endothelial Cells Equipment Design Robotics Fibroblasts Collagen Type I Extracellular matrix Equipment Failure Analysis chemistry.chemical_compound Micromanipulation Tissue engineering chemistry Polyethylene terephthalate Animals Humans Printing Cattle Computer Peripherals Cells Cultured Biomedical engineering |
Zdroj: | Tissue Engineering. 10:1566-1576 |
ISSN: | 1076-3279 |
Popis: | The primary emphasis of tissue engineering is the design and fabrication of constructs for the replacement of nonfunctional tissue. Because tissue represents a highly organized interplay of cells and extracellular matrix, the fabrication of replacement tissue should mimic this spatial organization. This report details studies evaluating the use of a three-dimensional, direct-write cell deposition system to construct spatially organized viable structures. A direct-write bioassembly system was designed and fabricated to permit layer-by-layer placement of cells and extracellular matrix on a variety of material substrates. Human fibroblasts suspended in polyoxyethylene/polyoxypropylene were coextruded through a positive displacement pen delivery onto a polystyrene slide. After deposition, approximately 60% of the fibroblasts remained viable. Bovine aortic endothelial cells (BAECs) suspended in soluble collagen type I were coextruded via microdispense pen delivery onto the hydrophilic side of flat sheets of polyethylene terephthalate. After deposition with a 25-gauge tip, approximately 86% of the BAECs were viable. When maintained in culture for up to 35 days, the constructs remained viable and maintained their original spatial organization. These results indicate the potential for utilizing a direct-write, three-dimensional bioassembly tool to create viable, patterned tissue-engineered constructs. |
Databáze: | OpenAIRE |
Externí odkaz: |