34S enrichment as a signature of thiosulfate oxidation in the 'Proteobacteria'
| Autor: | Aninda Mazumdar, Maida Jameela Rameez, Svetlana Fernandes, Subhrangshu Mandal, Sabyasachi Bhattacharya, A. Peketi, Wriddhiman Ghosh, Masrure Alam |
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| Rok vydání: | 2020 |
| Předmět: |
Chemoautotrophic Growth
Thiosulfates chemistry.chemical_element medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Paracoccus Proteobacteria Sulfur Isotopes Genetics medicine Sulfate Molecular Biology Betaproteobacteria Phylogeny 030304 developmental biology Tetrathionate Thiosulfate 0303 health sciences Paracoccus pantotrophus Pusillimonas ginsengisoli biology 030306 microbiology Sulfates biology.organism_classification Sulfur Kinetics chemistry Oxidation-Reduction Nuclear chemistry |
| Zdroj: | FEMS microbiology letters. 368(12) |
| ISSN: | 1574-6968 |
| Popis: | Kinetics of thiosulfate oxidation, product and intermediate formation, and 34S fractionation, were studied for the members of Alphaproteobacteria Paracoccus sp. SMMA5 and Mesorhizobium thiogangeticum SJTT, the Betaproteobacteria member Pusillimonas ginsengisoli SBO3, and the Acidithiobacillia member Thermithiobacillus sp. SMMA2, during chemolithoautotrophic growth in minimal salts media supplemented with 20 mM thiosulfate. The two Alphaproteobacteria oxidized thiosulfate directly to sulfate, progressively enriching the end-product with 34S; Δ34Sthiosulfate-sulfate values recorded at the end of the two processes (when no thiosulfate was oxidized any further) were −2.9‰ and −3.5‰, respectively. Pusillimonas ginsengisoli SBO3 and Thermithiobacillus sp. SMMA2, on the other hand, oxidized thiosulfate to sulfate via tetrathionate intermediate formation, with progressive 34S enrichment in the end-product sulfate throughout the incubation period; Δ34Sthiosulfate-sulfate, at the end of the two processes (when no further oxidation took place), reached −3.5‰ and −3.8‰, respectively. Based on similar 34S fractionation patterns recorded previously during thiosulfate oxidation by strains of Paracoccus pantotrophus, Advenella kashmirensis and Hydrogenovibrio crunogenus, it was concluded that progressive reverse fractionation, enriching the end-product sulfate with 34S, could be a characteristic signature of bacterial thiosulfate oxidation. |
| Databáze: | OpenAIRE |
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