Use of an Amplified-Fragment Length Polymorphism Technique To Fingerprint and Differentiate Isolates of Helicobacter pylori
| Autor: | Robert J. Owen, Eleanor R. Slater, D. S. Tompkins, Jacqueline Xerry, J. R. Gibson |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Adolescent Genotype Restriction Mapping Deoxyribonuclease HindIII Microbial Sensitivity Tests HindIII Polymerase Chain Reaction Helicobacter Infections Nuclear Family law.invention Restriction map law Humans Typing Child Polymerase chain reaction DNA Primers Genetics Base Sequence Helicobacter pylori biology Bacteriology Templates Genetic bacterial infections and mycoses Molecular biology Restriction enzyme Gastric Mucosa biology.protein Female Amplified fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 36:2580-2585 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.36.9.2580-2585.1998 |
| Popis: | Amplified-fragment length polymorphism (AFLP) analysis is the name given to a genotypic technique in which adapter oligonucleotides are ligated to restriction enzyme fragments and then used as target sites for primers in a PCR amplification process. The amplified fragments are electrophoretically separated to give strain-specific band profiles. We have developed a single-enzyme approach that did not require costly equipment or reagents for the fingerprinting of strains of Helicobacter pylori . The method was assessed with 46 isolates of H. pylori from 28 patients, and the results were compared with those from other genotypic tests. The AFLP profiles derived from Hin dIII fragments differentiated strains of H. pylori from unrelated individuals and confirmed the common origin of strains in some family members. AFLP analysis was also applied to investigate persistent infection following antibiotic therapy. Overall, the modified technique was relatively rapid and technically simple yet gave reproducible and discriminatory results. AFLP analysis samples variation throughout the genome and is a valuable addition to the existing genotypic fingerprinting methods for H. pylori . |
| Databáze: | OpenAIRE |
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