RADIATION-INDUCED PLASMID DNA DAMAGE: EFFECT OF CONCENTRATION AND LENGTH
| Autor: | Satoshi Kodaira, Václav Štěpán, Marie Davídková, Kateřina Pachnerová Brabcová, Martin Šefl, Zuzana Jamborová, Anna Michaelidesová |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA damage
030303 biophysics Linear energy transfer Polypropylenes Antioxidants 030218 nuclear medicine & medical imaging Ionizing radiation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Plasmid Radiation Ionizing Radiology Nuclear Medicine and imaging Heavy Ions Linear Energy Transfer 0303 health sciences Radiation Radiological and Ultrasound Technology Chemistry Public Health Environmental and Occupational Health Uncertainty Dose-Response Relationship Radiation General Medicine DNA PBR322 Dose–response relationship Gamma Rays Biophysics pUC19 Monte Carlo Method Algorithms DNA Damage Plasmids |
| Zdroj: | Radiation protection dosimetry. 186(2-3) |
| ISSN: | 1742-3406 |
| Popis: | Plasmid DNA is commonly used as a simpler substitute for a cell in studies of early effects of ionizing radiation because it allows to determine yields of primary DNA lesions. Experimental studies often employ plasmids of different lengths, in different concentrations in the aqueous solution. Influence of these parameters on the heavy-ion induced yields of primary DNA damage has been studied, using plasmids pUC19 (2686 bp), pBR322 (4361 bp) and pKLAC2 (9107 bp) in 10 and 50 ng/μl concentration. Results demonstrate the impact of plasmid length, while no significant difference was observed between the two concentrations. The uncertainty of the results is discussed. |
| Databáze: | OpenAIRE |
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