Photocleavage of Peptides and Oligodeoxynucleotides Carrying 2-Nitrobenzyl Groups
| Autor: | Brendan Manning, Raimundo Gargallo, Anna Aviñó, Roger Ramos, Ramon Eritja |
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| Rok vydání: | 2009 |
| Předmět: |
Oligodeoxynucleotide
DNA hairpin Biochemistry Catalysis Inorganic Chemistry chemistry.chemical_compound Drug Discovery Patterned surface Physical and Theoretical Chemistry Dna hairpin Aqueous solutions Melting curves Photocleavage Aqueous solution Oligonucleotide Chemistry Organic Chemistry Photodissociation Intermolecular force Oligodeoxynucleotides Combinatorial chemistry Duplex (building) Intramolecular force 2-nitrobenzyl DNA |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname ResearcherID CIÊNCIAVITAE |
| ISSN: | 1522-2675 0018-019X |
| DOI: | 10.1002/hlca.200800361 |
| Popis: | Peptides and oligodeoxynucleotides containing photolabile 2-nitrobenzyl groups as mid-sequences were prepared. Photocleavage of aqueous solutions of these compounds neared completion within 30 min to a few hours depending on the photolabile group used. A photolabile group was introduced in the loop of an intramolecular oligodeoxynucleotide hairpin. Melting curves of the hairpin with and without the complementary oligodeoxynucleotide showed a preference for the intramolecular hairpin form, but an intermolecular duplex was observed after photolysis. These results open the possibility of using photolabile DNA hairpins for the fabrication of patterned surfaces. |
| Databáze: | OpenAIRE |
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