Caffeine-loaded gold nanoparticles: antibiofilm and anti-persister activities against pathogenic bacteria
| Autor: | Young-Mog Kim, Seul-Ki Park, Nilushi Indika Bamunuarachchi, DoKyung Oh, Fazlurrahman Khan |
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| Rok vydání: | 2021 |
| Předmět: |
Multidrug tolerance
medicine.drug_class Antibiotics Metal Nanoparticles Microbial Sensitivity Tests medicine.disease_cause Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Listeria monocytogenes Caffeine Spectroscopy Fourier Transform Infrared medicine Escherichia coli 030304 developmental biology 0303 health sciences 030306 microbiology Pseudomonas aeruginosa Chemistry Biofilm Pathogenic bacteria General Medicine Anti-Bacterial Agents Staphylococcus aureus Biofilms Gold Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 105:3717-3731 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-021-11300-3 |
| Popis: | The formation of biofilms by bacterial pathogens and the presence of persister cells in biofilms have become major concerns in the health sector, owing to their antibiotic resistance and tolerance. The transformation of bacterial pathogens into persister cells, either stochastically or due to stressful environmental factors, results in recalcitrant and recurring infections. Here, we sought to prepare gold nanoparticles from naturally occurring caffeine and explore their inhibitory action against biofilm formation and persister cells. Fourier transform infrared spectroscopy, UV-visible absorption spectroscopy, field emission transmission electron microscopy, energy-dispersive X-ray diffraction, and dynamic light scattering were used to characterize the gold nanoparticles obtained from caffeine (Caff-AuNPs). The Caff-AuNPs were found to exhibit a number of properties, including the ability to prevent biofilm formation, disperse mature biofilms, and kill different types of persister of gram-positive (Staphylococcus aureus and Listeria monocytogenes) and gram-negative (Pseudomonas aeruginosa and Escherichia coli) pathogenic bacteria. Microscopic analysis of the aforementioned bacterial cells, treated with Caff-AuNPs, revealed the bactericidal effect of Caff-AuNPs, although the underlying mechanism remains unknown. Collectively, the Caff-AuNPs synthesized in this study may be used as potential drugs to combat chronic infections caused by biofilm-forming pathogenic bacteria. • Biofilm and persister cells are clinically relevant, as they either prolong or completely resist antibiotic treatments. • Caffeine is used in the green synthesis of Caff-AuNPs, which have antibacterial and antibiofilm properties. • Caff-AuNPs are effective against various pathogenic bacterial persister cells. |
| Databáze: | OpenAIRE |
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