Identification of Gal(beta 1-3)GalNAc bearing glycoproteins at the nodes of Ranvier in peripheral nerve
| Autor: | S. Apostolski, P. Chaliff, Norman Latov, A. Hays, Kari Stefansson, R. G. Lebaron, Massimo Corbo, Saud Sadiq, Arthur P. Hays, E. Ruoslahti, L. Suturkova‐Milosevic |
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| Rok vydání: | 1994 |
| Předmět: |
Peanut agglutinin
Blotting Western Molecular Sequence Data G(M1) Ganglioside Cellular and Molecular Neuroscience Myelin Peanut Agglutinin Western blot Lectins Ranvier's Nodes medicine Humans Amino Acid Sequence Peripheral Nerves Motor Neuron Disease Glycoproteins chemistry.chemical_classification biology medicine.diagnostic_test Lectin Molecular biology Immunohistochemistry Oligodendrocyte-Myelin Glycoprotein medicine.anatomical_structure chemistry Biochemistry Spinal Cord biology.protein Versican Glycoprotein Sequence Analysis Binding domain |
| Zdroj: | Journal of neuroscience research. 38(2) |
| ISSN: | 0360-4012 |
| Popis: | A subset of human anti-GM1 ganglioside antibodies cross-reacts with Gal(β1–3)GalNAc bearing glycoproteins in peripheral nerve and spinal cord. The same oligosaccharide determinant is recognized by the lectin peanut agglutinin (PNA) which binds at the nodes of Ranvier in intact peripheral nerve. The Gal(β1–3)GalNAc bearing glycoproteins were isolated using PNA lectin affinity chromatography followed by separation on Western blot, and the proteins were subjected to partial amino acid sequence analysis. Two major PNA binding glycoproteins were identified in peripheral nerve and spinal cord; one had an approximate molecular weight of 120 kD and had sequence homology to the oligodendrocyte-myelin glycoprotein (OMgp). The other migrated between 70 and 80 kD and had sequence homology to the hyaluronate binding domain of versican, which has been reported to share sequence homology with the 70 kD proteins hyaluronectin and the glial hyaluronic acid binding protein (GHAP). By immunocytochemistry, OMgp was localized to the paranodal region of myelin, and the protein homologous to the hyaluronate binding domain of versican was localized to the nodal gap in peripheral nerve. These PNA binding glycoproteins might be target antigens for autoantibodies in peripheral nerve. © 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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