Interactions of nuclear estrogen receptor with DNA and RNA
| Autor: | Susumu Ohno, Syr-Yaung Lin |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Receptor complex
medicine.drug_class Biophysics Estrogen receptor Oviducts Receptors Estradiol Biology Binding Competitive Biochemistry chemistry.chemical_compound Polydeoxyribonucleotides Structural Biology Genetics medicine Animals Nucleotide RNA Messenger Cell Nucleus chemistry.chemical_classification Estradiol RNA DNA Ribonucleotides Molecular biology Kinetics Receptors Estrogen chemistry Nuclear receptor RNA Ribosomal Estrogen Nucleic acid Female Chickens Polyribonucleotides Protein Binding |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 740:264-270 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/0167-4781(83)90135-5 |
| Popis: | The interactions of the nuclear estrogen receptor from hen oviduct with nucleic acids were studied by competition assay using DNA-cellulose centrifugation. We demonstrated that the estradiol-receptor complex binds similarly well to poly(A) RNA and denatured DNA. The estrogen receptor was found to interact more strongly with poly(G), poly(U) than with poly(A), poly(C). The receptor complex binds similarly to poly(A) and poly(dA), and to poly(U) and poly(dU). However, the receptor complex shows stronger binding to poly(G) than to poly(dG) and to poly(C) than to poly(dC). Studies with heteropolyribonucleotides indicated that poly(U 1 G 1 ) is more effective in competing for the estrogen receptor, and poly(AC) and poly(AUG) are moderately effective, whereas poly(ACU) is least effective. GMP and dGMP showed some competition for the nuclear receptor at 300-fold higher nucleotide concentrations than that of the synthetic poly(G). Observations that the nuclear estrogen receptor binds to poly(A) RNA and interacts selectively with polyribonucleotides suggest that the estrogen receptor-RNA interaction may play a role for the function of estrogens in gene regulation. |
| Databáze: | OpenAIRE |
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