Controlled enzymatic hydrolysis and synthesis of lignin cross-linked chitosan functional hydrogels
| Autor: | Gibson S. Nyanhongo, Bianca Beer, Lisa Berndorfer, Miguel Jimenez Bartolome, Günther Bochmann, Georg M. Guebitz |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Radical
Chemistry Techniques Synthetic macromolecular substances 02 engineering and technology Lignin Biochemistry Chitosan 03 medical and health sciences chemistry.chemical_compound Structural Biology Enzymatic hydrolysis Spectroscopy Fourier Transform Infrared Cellulose 1 4-beta-Cellobiosidase Lignosulfonates Molecular Biology Mechanical Phenomena 030304 developmental biology Laccase 0303 health sciences Hydrolysis technology industry and agriculture Hydrogels Free Radical Scavengers General Medicine 021001 nanoscience & nanotechnology Cross-Linking Reagents Solubility chemistry Self-healing hydrogels Molar mass distribution 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules. 161:1440-1446 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.08.030 |
| Popis: | This study presents a novel fully enzymatic process for the controlled depolymerisation of fungal and shrimp chitosan, and their subsequent use in the synthesis of lignin cross-linked chitosan (CTS) hydrogels. Cellobiosehydrolase (CBH) was used to depolymerize CTS resulting in decrease in average molecular weight (Mw) of shrimp CTS from 140 kDa and degree of deacetylation (DD %) from 91.3% to an average MW of 15 kDa and 16% DD. Similarly, fungal chitosan average molecular weight decreased from 92 kDa and the degree of deacetylation (DD) of 48.3% to 12 kDa and a DD of 13%. The depolymerized CTS were completely soluble in water and miscible with lignosulfonates without encountering the usual problem of formation of flocs. Introduction of laccase into a lignosulfonate-chitosan mixture resulted in the oxidation and generation of lignin reactive phenoxyl radicals that cross-linked with CTS-NH2 reactive groups resulting in the increase of Mw from 20 kDa to >500 kDa and viscosity from 20 mPa to >500 mPa. This resulted in the formation of stable lignin-cross-linked hydrogels with elongation at break of 111% and tensile strength of 7 mPa. The produced functional hydrogels have potential application in food and biomedical industries as e.g. as oxygen barriers in packaging or as functional wound dressing or tissue engineering platforms. |
| Databáze: | OpenAIRE |
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