| Autor: |
Michael A. Caligiuri, Pierluigi Porcu, James E. Bradner, Guido Marcucci, Jianying Zhang, Giandomenico Russo, Henry Wong, Jun Qi, Kathleen McConnell, Jing Wen, Douglas P. Curphey, Jessica Johns, Gregory H. Sams, Laura A. Sullivan, Sonya Kwiatkowski, Krista La Perle, Anjali Mishra |
| Rok vydání: |
2023 |
| DOI: |
10.1158/2159-8290.22531310.v1 |
| Popis: |
Supplementary Figures 1 - 7. Supplementary Figure 1. Immunoblot analysis of IL-15 in normal donor CD4+ T-cells, SS patient CD4+ T-cells and patient derived cell lines. Supplementary Figure 2. Representative flow cytometric histogram is shown for CD3 expression in mononuclear cells from skin of WT and IL-15 tg mice. Supplementary Figure 3. Immunoblot analysis of HDAC1 in normal donor CD4+ T-cells, SS patient CD4+ T-cells and patient derived cell line, Hut78. Protein lysates were probed for expression of HDAC1; β-Actin was used as housekeeping control. Supplementary Figure 4. Immunoblot analysis for expression of p21 protein in normal donor CD4+ cells following ten days of incubation in IL-15 compared to unstimulated normal donor CD4+ T-cells. Supplementary Figure 5. Pie chart showing the distribution of HDAC1 occupancy in normal donor CD4+ T-cells (upper left) compared to patient CD4+ T-cells (upper right) and normal donor CD4+ T-cells stimulated with 100ng/ml IL-15 for 24 hours (lower left). Supplementary Figure 6. High magnification and (B) low magnification histological analysis of IL-15 Tg skin. Supplementary Figure 7. The CTCL mouse disease severity scoring system is on a 0 to 100 scale, with 100 being the highest (most severe) score. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
